工业微生物2011，Vol.41Issue(5)：69-72,4.DOI:10.3969/j.issn.1001-6678.2011.05.015
阿拉伯胶酶产生菌株的筛选及其发酵培养基的优化
Screening of high producing strain for arabic gum degrading enzyme and its optimization of fermentation medium
摘要
Abstract
A fungi strain, which could produce arabic gum degrading enzyme, was screened out from soil, and then was identified as Fusarium sp. By the characteristic of its morphology of clone and spore. After mutagenesis by ultraviolet, a high-yielding strain designated ZHB05F was selected out. Further, the composition of fermentation medium for enzyme production was optimized. The results showed that the optimal fermentation medium was consisted of arabic gum 30 g/L, (NH4)2SO4 8 g/L, K2HPO4 1 g/L, MgSO4 ·7H2O 0.5 g/L, KC10.5 g/L, FeSO4 · 7H2O 0.01 g/L and H3BO3 0.01 g/L with pH 5～6, the activity of enzyme was up to 147.7U/mL produced by the strain ZHB05F.关键词
阿拉伯糖/镰刀菌/培养基优化Key words
arabinose/ Fusarium sp./ optimization of medium引用本文复制引用
陈志锋,廖青青,应国清,梅建凤,陈建澍..阿拉伯胶酶产生菌株的筛选及其发酵培养基的优化[J].工业微生物,2011,41(5):69-72,4.基金项目
浙江省科技计划项目(2009C14027). （2009C14027）
