中国动脉硬化杂志2011，Vol.19Issue(10)：813-818,6.
麝香保心丸对过氧化氢诱导人脐静脉内皮细胞凋亡及炎症因子表达的影响
Effect of Shexiang Baoxin Wan on Cell Apoptosis and Expression of Inflammatory Factors in Human Umbilicial Vein Endothelial Cells Induced with Hydrogen Peroxide
许昌声 1宁若冰 1柴大军 1林金秀1
作者信息
- 1. 福建医科大学附属第一医院心血管内科福建省高血压研究所,福建省,福州市,350005
- 折叠
摘要
Abstract
Aim To investigate the effect of Shexiang Baoxin Wan on cell proliferation, apoptosis and expressionof inflammatory factors in human umbilicial vein endothelial cells ( HUVEC) induced with hydrogen peroxide ( H2O2 ).Methods Collagenase I was used to digest and isolate HUVEC and then cultured. Bromodeoxyuridine ( Brdtl) enzyme-linked immunosorbent assay (El-IS A) was used to assay proliferation of HUVEC in vitro. Cell apoptosis was determined by terminal Dutp nick end tabling (TUNEL). Expression of monocyte chemotactic protein-1 ( MCP-1) , interleu-kin-6 (IL-6) and nuclear factor-kappa B (NF-Kb)-P65 Mrna was detected by reverse transcription polymerase chain reaction ( RT-PCR). Western-blotting was performed to detect the expression of NF-Kb-p65. Results (1) Compared with control, cell growth and proliferation was significantly lower in H2O, group. The serum of Shexiang Baoxin Wan could inhibit the HUVEC proliferation declined by H2O2 in concentration-dependent manner. Apoptosis was higher in H2O2 groups than that in control group and reserved to normal in 1 g Shexiang Baoxin Wan. (2)Compared with control, the level of MCP-1, IL-6 and NF-Kb Mrna in H2O2 group was greatly increased. Shexiang Baoxin Wan could inhibit theexpression of these inflammatory factors induced by H2O2 in concentration-dependent manner. The serum of 1 g Shexiang Baoxin Wan remarkly decreased the level of MCP-1 ,IL-6 and NF-Kb-P65 Mrna. (3) Compared to control,the level of NF-Kb-P65 was largely increased in H2O2 group, Shexiang Baoxin Wan could inhibit the expression of NF-Kb-P65 in concentration-dependent manner. The serum of 1 g Shexiang Baoxin Wan greatly decreased the level of NF-Kb-P65. Conclusion The role of Shexiang Baoxin Wan in protecting endothelial cell may be related to inhibiting the inflammatory factors induced by HO2.关键词
麝香保心丸/内皮细胞/炎症因子Key words
Shexiang Baoxin Wan/Endothelial Cells/Inflammatoiy Factors分类
医药卫生引用本文复制引用
许昌声,宁若冰,柴大军,林金秀..麝香保心丸对过氧化氢诱导人脐静脉内皮细胞凋亡及炎症因子表达的影响[J].中国动脉硬化杂志,2011,19(10):813-818,6.
