北京中医药大学学报2011，Vol.34Issue(7)：460-464,5.
基于小型猪冠心病心肌缺血模型的血瘀证蛋白组学研究
Proteomics of blood stasis syndrome based on myocardial ischemia model of coronary heart disease in minswines
摘要
Abstract
Objective To find the differential proteins of blood stasis syndrome of coronary heart disease (CHD) , and explore the possible internal pathogenesis of blood stasis syndrome. Methods The model of blood stasis syndrome of chronic myocardial ischemia was established by applying the method of placing Ameroid ring in the left anterior descending coronary artery in Chinese miniswines. All miniswines were randomly divided into the sham-operation group and model group. The status of myocardial ischemia (0-8 weeks) was observed dynastically in the model. The stenosis of coronary artery was observed by using coronary angiography ( CAC). The study of comparative proteomics was conducted by applying two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry ( MALDI-TOF-MS) in 10 miniswines of the sham-operation group and 9 ones of the model group. The relevant differential proteins were verified initially by using Western blot method at later stage of the test for determining further the differential proteins of CHD myocardial ischemia. Results The initial study found out that there were 5 distinct down-regulated protein spots and 8 distinct up-regulated protein spots in the model group compared with the sham-operation group. The identification ofMALDI-TOF-MS was used in some differential protein spots and the result was determined by second-order mass spectrum at the same time. The differential proteins were mainly oxidative stress proteins and relevant myocardial structural proteins, including heat shock 27 kDa protein ( HSP27 ) , peroxiredoxin 3 isoform 1, cardiac troponin T (cTnT) and myosin light polypeptide. Conclusion The myocardial manifestations were observed as oxidative stress lesion and changes of myocardial structural proteins in blood stasis syndrome of CHD chronic myocardial ischemia. The relevant differential proteins can be taken as the target of medicinal treatment.关键词
冠心病/慢性心肌缺血/血瘀证/蛋白组学/小型猪Key words
coronary heart disease/chronic myocardial ischemia/blood stasis syndrome/proteomics/miniswines分类
医药卫生引用本文复制引用
王勇,啜文静,郭淑贞,李春,王伟..基于小型猪冠心病心肌缺血模型的血瘀证蛋白组学研究[J].北京中医药大学学报,2011,34(7):460-464,5.基金项目
国家科技重大专项资助项目(No.2009ZX09502-018) （No.2009ZX09502-018）
国家自然科学基金资助项目(No.30902020) （No.30902020）
