广东药学院学报2011，Vol.27Issue(4)：389-391,3.DOI:10.3969/j.issn.1006-8783.2011.04.015
儿茶HPLC指纹图谱研究
Studies on HPLC fingerprints of Acacia catechu
摘要
Abstract
Objective To develop the fingerprints of Acacia catechu based on high performance liquid chromatography ( HPLC). Methods The HPLC separation was performed on a Hypersil ODS2 (150 mm x 4.6 mm,5 ^m) column by gradient eluting with methyl cyanide and 0.05% phosphoric acid at the flow rate of 1.0 Ml/min. The UV detection wavelength was set at 265 run,and the column temperature was maintained at 30 t. Similarity of fingerprint were calculated by sofware of " Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004A" ,and Samples were clustered by SPSS sofware. Results Twelve common peaks were identified and the similarity of 10 batches of Acacia catechu was more than 95%. Conclusion The fingerprints established could be used for the quality control of Acacia catechu due to its high stability and good reproducibility.关键词
儿茶/指纹图谱/HPLCKey words
Acacia catechu/ fingerprints/ HPLC分类
中医学引用本文复制引用
李锐,陈国彪..儿茶HPLC指纹图谱研究[J].广东药学院学报,2011,27(4):389-391,3.基金项目
国家科技支撑计划课题(2007 BAI27 B06) （2007 BAI27 B06）
