四川大学学报（医学版）2011，Vol.42Issue(5)：721-724,4.
抗hMOF单克隆抗体的制备与鉴定
Preparation and Identification of Monoclonal Antibody Against hMOF
孙厚良 1魏大鹏 2张小洪 2刘彦君 2章崇杰2
作者信息
- 1. 重庆三峡医药高等专科学校免疫学教研室 万州404016
- 2. 四川大学华西基础医学与法医学院免疫学教研室 成都610041
- 折叠
摘要
Abstract
Objective To prepare and identify the monoclonal antibody (mAb) against hMOF. Methods BALB/C mice were immunized with protein from the spleen cells isolated and fused with SP2/0 cells. After several rounds of screening and cloning, the hybridoma cell strain secreting anti-hMOF mAb was obtained. Its specificity was evaluated with ELISA and Western blot, and the titer, immunoglobulin subtype and affinity of the mAb were measured. Results One cell strains of hybridoma were obtained and named as 4C1C8. The anti-hMOF mAb secreted by the hybridoma cell strain was identified as IgGl subtype. The mAb titers in ascitic fluid were 1 * 409600, as determined with ELISA with an affinity reaching to 7. 65 X 106 L/mol. Western blot demonstrated that the antibodies could specifically recognize the immunogen. The cell immunohistochemistry proved that the antibody could recognize the hMOF antigens expressed on the normal cells HL7702. Conclusion The success in anti-hMOF mAb preparation provides the basis for further study of hMOF.关键词
hMOF/单克隆抗体/制备与鉴定Key words
hMOF/Monoclonal antibody/Preparation and identification引用本文复制引用
孙厚良,魏大鹏,张小洪,刘彦君,章崇杰..抗hMOF单克隆抗体的制备与鉴定[J].四川大学学报（医学版）,2011,42(5):721-724,4.
