中国药房2011，Vol.22Issue(31)：2894-2897,4.
HPLC法测定蝙蝠葛碱与大鼠和人血浆蛋白的结合率
Determination of Rat and Human Plasma Protein Binding Rate of Dauricine by HPLC
高秀蓉 1蒋学华 2王婷1
作者信息
- 1. 四川大学华西药学院临床药学与药事管理学系,成都市610041
- 2. 成都医学院药学院药剂教研室,成都市610083
- 折叠
摘要
Abstract
OBJECTIVE: To establish the HPLC method for the determination of dauricine, and to study the plasma protein binding rate of it. METHODS: The equilibrium dialysis and HPLC were used to determine the concentration of dauricine, and rat and human plasma protein binding rate of dauricine was calculated. RESULTS: The linear range of dauricine in extracellular fluid microdialysis was 0.24~15.10 μg·mL-1;in rat plasma was 0.24~15.10 μg·mL-1;in human plasma was 0.24-15.10 μg·mL-1;At high, middle and low concentrations, the rat plasma protein binding rates of dauricine were (69.63 ± 1.30)% , (68.64 ± 1.10)% , (72.54 ± 0.50)% , respectively. The human plasma protein binding rates of dauricine were (82.1 ± 1.00)% , (84.96 ± 0.70)% , (78.79 ±0.60)%, respectively. CONCLUSIONS: HPLC method is simple, sensitive and selective for the determination of biological sample of dauricine. The plasma protein binding rate of dauricine is of a little high, and the human protein binding rate is higher than that of rat, there is difference among species(P＜0.05).关键词
高效液相色谱法/蝙蝠葛碱/血浆蛋白结合率Key words
HPLC/Dauricine/Plasma protein binding rate分类
医药卫生引用本文复制引用
高秀蓉,蒋学华,王婷..HPLC法测定蝙蝠葛碱与大鼠和人血浆蛋白的结合率[J].中国药房,2011,22(31):2894-2897,4.
