中国药房2011，Vol.22Issue(35)：3288-3291,4.
金龙胆草总皂苷诱导Hela细胞和SPC-A1细胞凋亡的研究
Effects of Total Saponins from Conyza blinii on the Apoptosis of Hela Cells and SPC-A1 Cells
刘培 1周立军 2苏艳芳 1颜世伦1
作者信息
- 1. 天津大学药物科学与技术学院,天津市300072
- 2. 河北化工医药职业技术学院,石家庄市050026
- 折叠
摘要
Abstract
OBJECTIVE: To study the effects of total saponins from Conyza blinii on the proliferation of Hela cells and SPC-A1 cells. METHODS: The effect of total saponins from C. Blinii on the growth of Hela cells and SPC-A1 cells was observed by MTT assay. The change of DNA was detected by agarose gel electrophoresis and Western-blot assay was used to detect the effect of total saponins from C. Blinii on caspase-3 content of Hela cells and SPC-A1 cells. RESULTS: The MTT assay showed that the inhibition effect of 10-3~10-7 mol·L-1 total saponins from C. Blinii on the growth of Hela cells and SPC-A1 cells had clear dose and time dependence. Apoptotic cells had characteristic DNA ladder. The change of caspase-3 in cells had been observed. CONCLUSION : Total saponins from C. Blinii inhibit the growth of Hela cells and SPC-A1 cells by inducing cell apoptosis.关键词
金龙胆草总皂苷/Hela细胞/SPC-A1细胞/凋亡/半胱氨酰天冬氨酸特异性蛋白酶-3Key words
Total saponins from Conyza blinii/Hela cell/SPC-A1 cell/Apoptosis/Caspase-3分类
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刘培,周立军,苏艳芳,颜世伦..金龙胆草总皂苷诱导Hela细胞和SPC-A1细胞凋亡的研究[J].中国药房,2011,22(35):3288-3291,4.
