中国临床药理学与治疗学2011，Vol.16Issue(8)：856-861,6.
肉苁蓉总苷对原代培养小鼠肝细胞酒精损伤保护作用研究
Protective effect of cistanchis glycosides on ethanol-induced damage in the primary hepatocytes of mice
摘要
Abstract
AIM: To discuss the protection of cistanchis glycosides on ethanol-induced damage in the primary hepatocyte of mice. METHODS: The primary hepatocytes were collected by per-fusion in situ method. The cell livability was e-valuated by MTT, fluorescence microscopy and flow cytometry were used to evaluate the cell ap-optosis and necrosis, immunohistochemistry was used to detect the expression of bcl-2 and c-fos. RESULTS: After the primary hepatocytes were damaged by alcohol,the cell livability decreased, apoptosis and necrosis were obvious, the expression of bcl-2 was decreased while that of c-fos was increased. Cistanchis glycosides could increase the cell livability, ameliorate apoptosisand necrosis, increase the expression of bcl-2, and decrease the expression of c-fos in a dose-dependent manner. CONCLUSION: The protective effects of cistanchis glycosides on ethanol-induced liver damage in the primary hepatocytes is associated with increasing the expression of bcl-2, and decreasing the expression of c-fos; resulting less apoptosis, less necrosis, and increasing the cell livability.关键词
肉苁蓉总苷/原代培养/肝细胞/酒精性肝损伤/凋亡Key words
Cistanchis glycosides/ Primary hepatocyte/ Ethanol-induced liver damage/ Apoptosis分类
医药卫生引用本文复制引用
罗慧英,刘渊,杨焕..肉苁蓉总苷对原代培养小鼠肝细胞酒精损伤保护作用研究[J].中国临床药理学与治疗学,2011,16(8):856-861,6.基金项目
甘肃省教育厅科研项目(0906B-01) （0906B-01）
