中国临床药理学与治疗学2011，Vol.16Issue(8)：874-879,6.
榄香烯对B细胞淋巴瘤Raji细胞株的体内外抑制作用
In vivo and in vitro anti-tumor effects of elemene on B-cell lymphoma cell line Raji
摘要
Abstract
AIM: To investigate the inhibitory effects of elemene on B-cell lymphoma cell lineRaji in vitro and in vivo. METHODS: Raji cells were incubated with indicated concentrations ofelemene. The growth of Raji cells were observed by CCK-8 colorimetric assay, apoptosis rates were detected by flow cytometry using Annexin V-FITC/PI staining, analysis of nuclear morphology were observed with DAPI staining, cell cycles were analyzed by PI staining. And its in vivo anti-tumor effect on transplanted Raji cells in SCID mice was detected as well. RESULTS: Raji cells viability in presence of elemene decreased markedly in a dose and time dependent manner. The apoptosis could be induced by elemene and its level increased following the augmentation of the drug concentration. The characteristic apoptosis nuclei were observed in DA-PI staining after elemene treatment. A concentration dependent increase of cells in G0/G1 phase, a decrease in S phase and G2/M phase were detected. Elemene had a great ability to inhibit the growth of Raji cells in SCID mice. CONCLUSION: Elemene can significantly inhibit the growth of Raji cells, its function is performed by inducing apoptosis and arresting cell cycles in G0/G1 phase.关键词
榄香烯/Raji细胞/细胞凋亡/动物实验Key words
Elemene/ Raji cell/ Apoptosis/Animal experiment分类
医药卫生引用本文复制引用
徐希,庄彦,章圣辉,俞康..榄香烯对B细胞淋巴瘤Raji细胞株的体内外抑制作用[J].中国临床药理学与治疗学,2011,16(8):874-879,6.基金项目
浙江省自然科学基金资助项目(302781) （302781）
