中国普通外科杂志2011，Vol.20Issue(8)：835-858,24.
IFN-γ对小鼠心脏移植免疫耐受的影响
The impact of IFN-γ on cardiac transplant tolerance in mice
摘要
Abstract
Objective To investigate the impact of IFN-γ on cardiac transplant tolerance induced by blockade of CD40-CD40 ligand costimulation pathway in mice. Methods IFN-γ expression in cardiac grafts and spleens from syngeneic and allogeneic recipients with or without treatment of anti-CD40 ligand monoclonal antibody ( MR-1 ) was examined by realtime RT-PCR. The survival time of cardiac grafts in Wild type and IFN-γ-deficient recipients was investigated. Mixed lymphocyte reaction ( MLR) of CD4+ T cells and cytotoxic T lymphocyte assay of CD8+ T cells from Wild type recipients and IFN-γ-deficient recipients administrated with MR-1 were also studied. Results Rejected cardiac allogafts showed significantly higher expression of IFN-'y than tolerant allogafts. Cardiac allograft survival was not prolonged in nonimmunosuppressed IFN-γ-deficient mice. In fact, graft survival time in IFN-γ-deficient mice was somewhat shorter than that observed in Wild type recipients. Administration of MR-1 induced long-term cardiac allograft survival in Wild type recipients, but failed to do so in the IFN-γ-deficient group. Our results also provided evidence that in vivo absence of IFN-γ in recipients facilitated the proliferation and CTL generation of T cells. Conclusions IFN-γ faciliates the formation of transplant tolerance induced by blockade of CD40-CD40 ligand costimulation pathway.关键词
γ干扰素/共刺激信号/小鼠心脏移植/免疫耐受Key words
IFN-γ/Costimulation Pathway/Mouse Heart Transplantation/Tolerance分类
医药卫生引用本文复制引用
姜晓峰,林琳,朱磊,崔哲铭,郭大伟,梁健..IFN-γ对小鼠心脏移植免疫耐受的影响[J].中国普通外科杂志,2011,20(8):835-858,24.基金项目
教育部留学回国人员科研启动基金资助项目(2008890) （2008890）
辽宁省教育厅高等学校科研项目计划(2008824). （2008824）
