四川大学学报（医学版）2011，Vol.42Issue(5)：599-603,615,6.
甘露聚糖结合凝集素阻遏人巨细胞病毒感染MD-DC细胞的研究
Mannose-binding Lectin (MBL) Inhibits Human Cytomegalovirus Infection of Human MD-DC
摘要
Abstract
Objective To explore the inhibitory effect of different sources, different concentrations of Mannose-binding lectin ( MBL) on human cytomegalovirus infection of human MD-DC cells. Methods The recombinant MBL was acquired by vector construction, and the natural MBL was purified from human plamsa. MD-DC were pre-exposed to several dilutions of the hMBL/rMBL for 30 min, then HCMV suspensions were added to MD-DC for 2 h to compare the inhibitory effect of hMBL/rMBL on the HCMV infection of MD-DC. MD-DC infected by HCMV co-culture with hMBL/rMBL to compare the inhibitory effect of hMBL/rMBL on the HCMV diffusion between MD-DC. HCMV-DNA in MD-DC was detected by fluorescence quantitative PCR. HCMV-PP65 in MD-DC was analyzed with flow cytometry, the ability of MD-DC to capture HCMV was observed with imtnunofluorescence confocal microscope. Results In hMBL/rMBL inhibition the ability of MD-DC capture HCMV experiments, the fluorescent quantitative PCR demonstrated that the amount of HCMV-DNA in 1 μg/mL of hMBL/ rMBL treated cells was not significantly different from that of control group (P<0. 05). But the HCMV-DNA in 5 jig/mL and 10 fig/mL hMBL/rMBL treated group were significantly lower than that of control group (P<0. 05). The significant inhibit effects of 10 fig/mL hMBL / rMBL on the ability of MD-DC capture HCMV were observed by immunofluorescence confocal microscopy and flow cytometry. The inhibit effects of hMBL/rMBL on HCMV diffusion between MD-DC were also observed in 5 μg/mL and 10 μg/mL hMBL/rMBL treated groups at 72 hours. Conclusion The hMBL/rMBL in physiological concentration range (5-10 μg/mL) can significantly inhibit human cytomegalovirus infection of human MD-DC cells, and the hMBL is more effective than rMBL.关键词
人巨细胞病毒/甘露聚糖结合凝集素/MD-DC/阻遏/感染Key words
HCMV/MBL/MD-DC/Inhibit/Infection引用本文复制引用
潘开宇,尚世强,陈英虎,吴蔚,乔会菊,陶然..甘露聚糖结合凝集素阻遏人巨细胞病毒感染MD-DC细胞的研究[J].四川大学学报（医学版）,2011,42(5):599-603,615,6.基金项目
国家自然科学基金(批准号30872240)资助 （批准号30872240）
