四川大学学报（医学版）2011，Vol.42Issue(5)：610-615,6.
过氧化氢诱导的人正常和早老细胞中DNA损伤及其修复研究
The Study of Hydrogen Peroxide Induced DNA Damage and Recovery in Normal Aging and Premature Aging Human Cells
摘要
Abstract
Objective To study the DNA damage and recovery induced by hydrogen peroxide in normal aging and premature aging human cells. Methods The immunofluorescent assay and comet assay were used to estimate basal DNA damage in normal aging BJ cells and premature aging Hutchinson-Gilford progeria syndrome (HGPS) cells, which were divided into three and two distinct population doubling (PD) number groups (BJ 14· 30, 45 and HGPS 8, 17) respectively. The DNA damage induced by hydrogen peroxide of these cell populations, as well as their repair activity, was also studied. Finally, the recovery capability before and after the xeroderma pigmentosum group A (XPA ) knocked down in these groups was measured. Results Our results indicated that the normal BJ cells of older PD number showed higher basal levels of DNA damage and were more sensitive to the effects of the DNA-damaging agents than the adult one, who, in turn, was more sensitive than the younger ones. The basal damage level of HGPS cells was same or higher than the older BJ cells. HGPS cells also had the same consistent damage change as BJ cells after treatment. The decline of the repair efficiency with age to the DNA damage induced by the agent was also observed. Conclusion The DNA repair of HGPS is repressed by dysfunction of XPA.关键词
彗星电泳/免疫荧光/过氧化氢/DNA损伤/DNA修复Key words
Comet assay/Immunofluorescent assay/Hydrogen peroxide/DNA damage/DNA repair引用本文复制引用
所起凤,杜文婷,杨鸣鸣,范雪娇,刘戟..过氧化氢诱导的人正常和早老细胞中DNA损伤及其修复研究[J].四川大学学报（医学版）,2011,42(5):610-615,6.基金项目
教育部博士点基金新教师项目(项目代码:20070610124)和教育部留学回国人员启动基金项目(项目代码:2008890-19-11)资助 （项目代码:20070610124）
