四川大学学报（医学版）2011，Vol.42Issue(5)：633-637,5.
胰腺癌差异蛋白质组学研究
Comparative Proteomics of Pancreatic Cancer
摘要
Abstract
Objective To identify differentially expressed proteins between pancreatic tumor tissues and the adjacent noncancerous tissues by comparative proteomics analysis. Methods Six pairs pancreatic tumor tissues and the adjacent noncancerous tissues were obtained and selected for two-dimensional polyacrylamide gel electrophoresis (2-DE) analysis. The differentially expressed proteins were identified using the PDQuest 2-D analysis software. After cutting and enzymolysis, the differentially expressed proteins were analysised by ESI-Q-TOF mass spectrometer. The MS/MS data were acquired and searched in the Swiss-Prot database using MASCOT software. Altered expression of representative proteins was validated by immunohistochemical staining. Results A total of 97 points were identified as differentially expressed by two-dimensional gel electrophoresis, of which 31 were successfully identified by ESI-Q-TOF mass spectrometer. Among the proteins identified by mass spectrometer, 23 were upregulated and the other 8 were downregulated. These proteins were involved in different functional processes and protein families: glucolysis, hyaluronidase, I , II -phase metabolic enzymes, metalloproteinase, cytoskeleton, Ca2+ metabolism and so on. Immunohistochemical staining revealed an overexpression of HYAL1 and low expression of CYP2C8 and GSTM2 in pancreatic tumor. Conclusion The differentially expressed proteins between pancreatic tumor tissues and the adjacent noncancerous tissues can be identified by comparative proteomics approach. The selected proteins will provide a valuable clue and help for further research.关键词
胰腺癌/蛋白质组学Key words
Pancreatic cancer/Proteomics引用本文复制引用
苏安平,李振军,田伯乐,曹双双,张懿,王振亮,胡韬,Shan Ram Babu,王维国..胰腺癌差异蛋白质组学研究[J].四川大学学报（医学版）,2011,42(5):633-637,5.基金项目
四川省科技厅科研基金(2009SZ0158)资助 （2009SZ0158）
