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纤维素同步糖化发酵生产乙醇

孙武举 翁海波 李萍萍 晋果果

安徽农业科学2011,Vol.39Issue(18):11018-11021,11126,5.
安徽农业科学2011,Vol.39Issue(18):11018-11021,11126,5.

纤维素同步糖化发酵生产乙醇

Research on the Ethanol Produced from the Simultaneous Glycation and Fermentation of Cellulose

孙武举 1翁海波 1李萍萍 1晋果果1

作者信息

  • 1. 郑州大学生物工程系,河南,郑州,450001
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摘要

Abstract

[Objective] The ethanol was produced by means of microbiological processes for the replacing approach of energy source. [ Method] The reducing sugar was produced from the cellulose, which was saccharified by the enzyme that was from the cellulose as raw material was acted by Aspergillus teneus-Mll,,and the ethanol was produced based on the fermentation of yeast. [ Result] The experimental result indicated that the optimal culture condition of Aspergillus terreus-Mll growth was that the best time was 3 days,the best inoculation was 200 μl,the optimal culture humidity was 80% ,the optimum temperature was 45℃ and the optimal culture pH was 3.0,under which condition,the largest amount of enzyme-producing was available. And the experiment in the glycation process of cellulase showed the optimal temperature was 55 ℃ and the optimum pH was 5.0,under which condition the production of reducing sugar,which enzyme activity under acidic condition was higher and had good thermal stability,was relevantly high. By fermentation,the reduced sugar accounted for 62.42% of the dry weight of raw material and the produced ethanol accounted for 21.36% of dry weight of raw material. [Conclusion] This method could be used for the production of ethanol at the basis of industrial fermentation with die environmental protection,cost-reducing and high social benefit,which application value was very good.

关键词

同步糖化发酵/土曲霉M11/乙醇/废纸/酶活性

Key words

Simultaneous glycation and fermentation/Aspergillus terreus/Ethanol/ Waste paper/ Activity

分类

生物科学

引用本文复制引用

孙武举,翁海波,李萍萍,晋果果..纤维素同步糖化发酵生产乙醇[J].安徽农业科学,2011,39(18):11018-11021,11126,5.

基金项目

河南省重点科技攻关资助项目(072102220002). (072102220002)

安徽农业科学

0517-6611

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