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水貂MLPH基因第一外显子序列结构及特征分析

张敏

安徽农业科学2011,Vol.39Issue(18):11097-11099,3.
安徽农业科学2011,Vol.39Issue(18):11097-11099,3.

水貂MLPH基因第一外显子序列结构及特征分析

Sequence Structure and Characteristics of Exon 1 of Mink MLPH Gene

张敏1

作者信息

  • 1. 辽宁医学院锦州畜牧兽医学院,辽宁,锦州,121001
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摘要

Abstract

[Objective] To clone exon 1 and its flanking sequences of MLPH gene from minks raised in western Liaoning region and to predict its structure. The study will provide a theoretical basis for breeding of colored mink. [ Method] The exon 1 and its 5'-flanking sequences of MLPH gene were amplified from minks. SignalP 3.0 Server,ClustalX 1.83,Mega 4.1,TMHM 2.0,ProtComp - v6.0 and other software were used for sequence analysis. [Result] A 1 029 bp fragment was amplified with the expected size. As calculated with the Mega 4.1 ,the minks had the nearest genetic distance with rattus (0.540 5) and the farthest with human (0.655 2). Phylogenetic tree of mink was constructed. [Conclusion] The Mink MLPH gene encodes a secretory protein.

关键词

水貂/MLPH基因/序列结构

Key words

Minks/VMLPH gene/ Sequence structure

分类

农业科技

引用本文复制引用

张敏..水貂MLPH基因第一外显子序列结构及特征分析[J].安徽农业科学,2011,39(18):11097-11099,3.

基金项目

辽宁医学院博士启动资金资助. ()

安徽农业科学

0517-6611

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