安徽农业科学2011，Vol.39Issue(18)：11106-11107,2.
柔嫩艾美耳球虫子孢子抗原的制备
Preparation of Sporozoite Antigen of E.tenella
摘要
Abstract
[objective] To provide antigen for preparation of monoclonal antibodies against E. Tenella. [ Method] Broiler chickens were inoculated with axenic Culture of sporulated oocysts of E. Tenella, and their feces were collected after 5 - 10 d. After simple separation, the oocysts were sporulated, purified and counted to prepare sporozoite antigen. [Result] This method used to prepare sporozoite antigen was simpler and easier than other methods. In addition, it required few specialized equipment and media, and it could be conducted in general laboratories. However, it needed long time. [Conclusion] The E. Tenella oocysts have been isolated from chickens, and the sporozoite antigen with high protein concentration has been obtained.关键词
柔嫩艾美耳球虫/子孢子抗原/制备Key words
E. Tenella/ Sporozoite antigen/ Preparation分类
农业科技引用本文复制引用
李巍,王丹,王哲,凡敏,张颖,赵权..柔嫩艾美耳球虫子孢子抗原的制备[J].安徽农业科学,2011,39(18):11106-11107,2.基金项目
国家自然科学基金资助项目(30972176). （30972176）
