植物研究2011，Vol.31Issue(5)：585-591,7.
白花泡桐种源的遗传多样性和遗传分化研究
Genetic Diversity and Genetic Differentiation for Paulownia fortunei Provenances
摘要
Abstract
Thirty-eight provenances of Paulownia fortunei were used to detect the genetic diversity and genetic differentiation by inter-simple sequence repeats (ISSR) analysis. The results showed that: (1)A total 95 DNA fragments were amplified using 9 primers with unambiguous unique polymorphic hands screened from 100 ISSR primers, polymorphic loci of which were 88 ( PPB = 92.63% ). (2) At the species level, the mean values of effective number of alleles (Ne) , Nei's gene diversity index (H) and Shannon's Information index (I) among provenances were 1. 391 0, 0. 242 4 and 0. 376 5, respectively, the percentages of polymorphic loci ( PPL) a-mong different provenances were between 32.63% (Fuzhou, Jiangxi) and 56. 84% (Wu Zhou, Guangxi and Jiu Jiang, Jiangxi) with an average of 47.16% ; Gene flow among populations (Nm) was 0.9127, the coefficient of population differentiation (G,,) was 0.353 9, which estimated that the genetic variation between provenances account for 35.39% , indicating that the genetic variation between provenances was higher than that of within provenances. (3) Genetic identity ranged from 0. 39 to 0. 82, reflecting the relatively wide genetic basis among P. Fortunei provenances. Thirty-eight provenances of P. Fortunei could be divided into 3 major groups obviously based on the UPGMA cluster analysis. A rotated PCoA plot with the markers as observations was constructed to determine 4 big groups. Principal coordinates analysis ( PCoA) for ISSR data did not give the same result with the UPGMA cluster, the corresponding discussion was made in the paper. The test also illustrated that there was no significant correlation between genetic and geographic distances among provenances.关键词
白花泡桐/遗传多样性/遗传分化/聚类分析/主坐标分析Key words
Paulownia fortunei/ genetic diversity/ genetic differentiation/ cluster analysis/ principal coordinates analysis分类
农业科技引用本文复制引用
莫文娟,袁德义,李芳东,乔杰,李荣幸,罗健,王路红..白花泡桐种源的遗传多样性和遗传分化研究[J].植物研究,2011,31(5):585-591,7.基金项目
国家林业公益性科研专项——泡桐基因库与育种群体建立技术研究(200804021) （200804021）
