中国动物传染病学报2011，Vol.19Issue(2)：13-19,7.
猪源TTV Taqman实时荧光定量PCR检测方法的建立和应用
DEVELOPMENT AND APPLICATION OF TAQMAN REAL-TIME FLUORESCENCE QUANTITATIVE PCR ASSAY FOR DETECTION OF PORCINE TORQUE TENO VIRUS
摘要
Abstract
Two Taqman real-time fluorescence quantitative PCRs were established using two sets of primers and probes based on the conserved untranslated region（UTR）of porcine Torque Teno virus genotype 1 and 2（TTV1 and TTV2）.The UTR sequences of TTV1 and TTV2 were cloned into the pMD18-T vector and a series of diluted recombinant plasmids were used to generate standard curves.The quantitative PCR assays could detect as less as 10 copies of target DNA of TTV1 or TTV2.The assay showed good specificity and did not cross react with other porcine viruses（PRRSV,CSFV,PCV2 and SIV）.The variation coefficient of intra-batch or inter-batch were both below 3%,which indicated good reproducibility.44 clinical samples of pigs from Neimeng and Guangxi were detected by the two quantitative PCR assays,and the results showed that 47.73%,70.45% and 31.82% of the sample were positive for TTV1,TTV2,and both,respectively.关键词
TTV1/TTV2/Taqman实时荧光定量PCRKey words
TTV1/TTV2/Taqman real-time fluorescence quantitative PCR分类
农业科技引用本文复制引用
侯军委,周艳君,王礞礞,李国新,于海,童光志..猪源TTV Taqman实时荧光定量PCR检测方法的建立和应用[J].中国动物传染病学报,2011,19(2):13-19,7.基金项目
上海市科技人才计划项目（09×D1405400) （）
国家生猪现代产业技术体系建设项目（MYCYTX-009) （）
