草业科学2011，Vol.28Issue(7)：1320-1325,6.
干旱胁迫下赖草抑制性消减文库的构建与分析
Construction of suppression subtractive hybridization library of Leymus secalinus exposed to drought stress
摘要
Abstract
The drought stress method of root without water was used to construct the subtracted cDNA li- brary of Leymus secalinus containing 576 clone by the method of suppression subtractive hybridization. In this study, the leaves under drought stress were considered as a tester and the leaves under normal condi- tion were considered as a driver. The dot blot hybridization and sequencing was applied to discover the 16 EST with length 100--310 bp. The searching against GenBank with blast algorithm showed that these se- quences were high homologous to the gene sequence of wheat and barley under drought stress, the gene se- quence of LcBADH2, the gene sequence of hsp70 of Oryza sativa, the gene sequence of RubisCO of Hordeurn vulgate, the amino acid sequence of Ubiquitin-conjugating enzyme of Zea mays, the amino acid se- quence of L20 of 50S Ribosomal protein of plant. This study suggested that these known genes and pro- teins play an important role in controlling plant osmotic pressure, heat adjustment, photosynthesis and hy- drolysis, and had established a basis for disclose the mechanism of drought resistance and cloning stress-resistance genes of Leymus secalinus.关键词
赖草/抑制性消减杂交/干旱胁迫/ESTKey words
Leymus secalinus/suppression subtractive hybridization/drought stress/EST分类
农业科技引用本文复制引用
杨满业,王丽焕,叶煜辉,江明锋,肖冰雪..干旱胁迫下赖草抑制性消减文库的构建与分析[J].草业科学,2011,28(7):1320-1325,6.基金项目
基金项目：四川省科技厅应用基础研究项目 （）
