中国兽医科学2011，Vol.41Issue(7)：723-727,5.
猪伪狂犬病病毒环介导等温扩增检测方法的建立
Establishment of a loop-mediated isothermal amplification assay for detection of pseudorabies virus
摘要
Abstract
The objective of this study was to establish a loop-mediated isothermal amplification（LAMP） method for detection of pseudorabies virus（PRV）.Four primers were designed according to the PRV gE gene sequence.Then the tests of specificity,sensitivity and reproducibility were carried out.In results,the developed system was specific for the detection of PRV,and there was no cross-reactivity with porcine reproductive and respiratory syndrome virus,porcine parvovirus,classical swine fever virus,swine influenza virus,pseudorabies virus with gE gene deleted.The sensitivity of this assay was 100 copies plasmid DNA and 10 times more sensitive than conventional PCR.These results showed that the LAMP me-thod had higher sensitivity,specificity and reproducibility,and could be used for the detection of PRV.关键词
伪狂犬病病毒/环介导等温扩增/检测Key words
pseudorabies virus/loop-mediated isothermal amplification/detection分类
农业科技引用本文复制引用
张莉,鄢明华,王东,鲍士国,韩伟,任卫科..猪伪狂犬病病毒环介导等温扩增检测方法的建立[J].中国兽医科学,2011,41(7):723-727,5.基金项目
天津市应用基础及前沿技术研究计划项目（09JCYBJC14900) （）
