果树学报2011，Vol.28Issue(4)：597-602,6.
山葡萄（双丰）转色期果皮cDNA文库构建和分析
Construction and analysis of cDNA library from Vitis amurensis grapevine skins at veraison
摘要
Abstract
With the aim to investigate the expression pattern of genes at different ripening stages so as to select the anti-insect,disease-resistant and high-yield genes,the veraison skin（50% colored） of Vitis amurensis was used as experimental material to construct the cDNA library.The pDNR-LIB was used as the vector and the recombinant plasmid was transformed into E.coli DH5α by the electroporation according to the CreatorTM SMARTTM Library construction Kit.The fragment size inserted was 0.5 to 2.0 kb and the average insert size was 0.80 kb.710 unigenes obtained were clustered into 97 contigs and 613 singlets.The result of the BlastX analysis indicated that there were 528 ESTs with known or unknown functions,182 ESTs with no significant similarity matching any protein or DNA sequence in the database.Some genes involved in the process of anthocyanin synthesis and defense/disease-resistant genes were detected in this library,such as glutathione S-transferase,zinc finger protein,chalcone synthase,and anthocyanidin 3-O-glucosyltransferase,which inferred that these genes were expressed actively at the veraison stage.关键词
山葡萄/cDNA文库/转色期/EST测序Key words
Vitis amurensis/cDNA library/Veraison/EST sequencing分类
农业科技引用本文复制引用
李波,杨成君,刘海峰,李凤,段长青,王军..山葡萄（双丰）转色期果皮cDNA文库构建和分析[J].果树学报,2011,28(4):597-602,6.基金项目
现代农业产业技术体系建设专项资金(nycytx-30) （nycytx-30）
