环境工程学报2011，Vol.5Issue(8)：1902-1906,5.
土壤中好氧反硝化菌的分离及脱氮特性研究
Screening aerobic denitrifiers from soil and study on characteristics of denitrification
王有乐 1雷兴龙 1陈连军 1杨勤 1王亚变1
作者信息
摘要
Abstract
An aerobic denitrifying bacterial strain name as LD3 was isolated from soil by using a new screening method.Samples were enriched by limiting diluted firstly,secondly screened with Griess and Diphenylamine reagent,then streak plate.The gram stain of the strain was positive,the cells growth cycle was rod or spherical,the clones of the strain were smooth,wet and white,the strain LD3 was identified as genus pimelobacter.Strain LD3 could denitrify nitrate companying with the formation of potassium nitrate in aerobic environment.The denitrifying capability of the strain was studied in batch culture under aerobic condition.The results showed that the nitrate in the culture could be efficiently removed by strain LD3 and the nitrogen removal rate was up to above 97.67%.The factors affecting aerobic denitrificatrion by strain LD3 were also discussed.The results indicated that strain LD3 had a wider spectrum of carbon and nitrogen source except for high concentrations of nitrite inhibiting the growth of the strains,the optimal C/N ratio and pH value for aerobic denitrification were 8 and about 7.5,the most suitable temperatures for aerobic denitrification were 30~35 ℃.关键词
土壤/好氧反硝化菌/筛选/脱氮特性Key words
soil/aerobic denitrifier/screening/characteristics of denitrification分类
资源环境引用本文复制引用
王有乐,雷兴龙,陈连军,杨勤,王亚变..土壤中好氧反硝化菌的分离及脱氮特性研究[J].环境工程学报,2011,5(8):1902-1906,5.
