海洋科学2011，Vol.35Issue(7)：7-13,7.
文昌鱼线粒体基因组特征分析及分子标记探讨
Analysis of the characteristics of mitochondrial genomes and exploration of molecular markers in amphioxus
摘要
Abstract
Mitochondrial genomes have been widely used in metazoan molecular phylogeny and genetic research. Amphioxus, as research model animals, occupies an important position in the origin and evolution of vertebrates. The basic characteristics of amphioxus mitochondrial genomes were fully revealed by our comprehensive analysis of 51 mitochondrial genome sequences from seven species. Amphioxus mitochondrial genomes contain 37 standard metazoan genes. Three types of gene arrangements were found in amphioxus mitochondrial genomes. Gene arrangements of Branchiostoma and Epigonichthys are most similar to those of typical vertebrate mitochondrial genomes. Therefore, gene arrangement shared by Branchiostoma and Epigonichthys represents the ancestral amphioxus gene order. In contrast, gene rearrangements were found in three species of the genus Asymmetron. The Ka/Ks ratios of the 13 amphioxus mitochondrial protein-coding genes were much lower than one （range between 0 and 0.3363）, indicating a strong purifying selection （negative selection）. The genetic variation analysis of main genes （13 protein coding genes and two ribosomal RNA genes） among and within amphioxus species shows that genes had5, had4 and had2 are ideal molecular markers supplementary to the coxl gene, and will provide useful information on conservation of amphioxus biological diversity and the utilization of biological resources.关键词
文昌鱼（amphioxus）/线粒体基因组/差异位点/分子标记Key words
Amphioxus/mitochondrial genome/genetic different loci/molecular marker分类
生物科学引用本文复制引用
申欣,田美,孟学平,程汉良..文昌鱼线粒体基因组特征分析及分子标记探讨[J].海洋科学,2011,35(7):7-13,7.基金项目
国家自然科学基金资助项目 （）
江苏省青蓝工程人才基金资助项目 （）
江苏省海洋生物技术重点建设实验室研究基金资助项目 （）
淮海工学院自然科学基金资助项目 （）
