海洋科学2011，Vol.35Issue(7)：26-29,36,5.
海洋贝类附生细菌区系分析及弧菌拮抗菌的筛选
Isolation and biodiversity analysis of bacteria attached on the marine shellfish and selection of vibrios-antagnism bacteria
摘要
Abstract
A preliminary screening of bacteria attached on the marine shellfish from six marine shellfishes（Mytilus edulis, Concha ostreae, Ruditapes variegate, Anadara uropygimelana, Placopecta magellanicus, and Ovula ovum） in the coastal waters of Weihai yielded 100 isolates, among which 45 strains were selected for further analysis. To investigate the phylogenetic position of these strains, the 16S rDNA sequences were cloned, sequenced, and com- pared with those of related strains and a molecular phylogenetic dendrogram was constructed based on genetic dis- tance analysis. The strains were classified as members of genera Arthrobacter, Bacillus, Bizionia, Brevundimonas, Cellulophaga, Cobetia, Gelidibacter, Kocuria Krokinobacter, Lacinutrix, Marinobacter, Microbacterium, Micrococcus, Paracoccus, Planomicrobium, Pseudoalteromonas ,Psychrobacter, Salinibacterium, Shewanella, Tenaci- baculum, and Vibrio. The antimicrobial activity of the 100 strains were screened by the agar flat seeding method. The experiment results showed that 2 strains had obvious antimicrobial activity.关键词
细菌多样性/海洋贝类/16S/rDNA/区系分析/拮抗Key words
bacterial diversity/marine shellfish/16S rDNA/flora analysis/antagonistic分类
生物科学引用本文复制引用
张柯,丁翠玲,张栋..海洋贝类附生细菌区系分析及弧菌拮抗菌的筛选[J].海洋科学,2011,35(7):26-29,36,5.基金项目
山东大学威海分校科研立项资助项目 （）
