果树学报2011，Vol.28Issue(4)：721-726,6.
草莓EST-SSR标记的开发与应用
Development and utilization of EST-SSR marker in strawberry
摘要
Abstract
61 731 ESTs（Expressed Sequence Tags） of strawberry were downloaded from public databases,14948 non-redundant ESTs were assembled,the total length of these non-redundant ESTs was 9 969.35 kb.1 807 SSRs（Simple Sequence Repeats） distributed in 1 515 ESTs were mined,the appearance frequency was 10.14%,one average SSR was found per 5.52 kb.235 ESTs contained two or more than two SSRs,compound form was shown in 76 ESTs.Dinucleotides（794 SSR） and trinucleotides（795 SSR） EST-SSRs were dominant,accounted for 43.94%,44.00% respectively.AG/CT and AAG/CTT were major motifs between dinucleotides and trinucleotides.The frequency was 38.24% and 15.38%,respectively.Using these sequences,47 primer pairs were designed and tested in 3 major Japanese cultivars for farmers,44 primer pairs could be amplified,6 primer pairs revealed polymorphisms.The results showed that the primers which developed from EST databases were efficient and practicable and could be used for strawberry cultivar identification,germplasm analysis and linkage mapping.关键词
草莓/EST-SSR/标记开发Key words
Strawberry/EST-SSR/Marker development分类
农业科技引用本文复制引用
王静,赵密珍,于红梅,王壮伟..草莓EST-SSR标记的开发与应用[J].果树学报,2011,28(4):721-726,6.基金项目
农业部行业计划(201003064) （201003064）
江苏省科技基础设施建设计划(BM2008008) （BM2008008）
