果树学报2011，Vol.28Issue(4)：689-693,5.
龙眼LEAFY同源基因启动子的克隆与序列分析
Cloning and sequence analysis of LEAFY gene promoter from longan（Dimocarpus longan）
摘要
Abstract
In order to study the expression and regulation rule of LEAFY homolog in longan（LLFY）,a 809 bp fragment of LLFY promoter was cloned by chromosome walking method.Promoter sequence analyzed by PlantCARE and PLACE showed that this promoter fragment had TATA-BOX,CAAT-BOX and some other regulatory elements,such as cis-acting element involved in circadian control,MYB binding site involved in drought-inducibility,cis-acting regulatory element involved in the abscisic acid responsiveness and light responsive element.It was supposed that the expression of LLFY was regulated by circadian,drought,light et al.Compared the promoter of LLFY with other 5 plants by FootPrinter analysis,these promoters had conserved domains while the distribution of transcription factor-binding site had difference.It implied the diversity of gene function of LEAFY genes,and LLFY may had different functions.关键词
龙眼/LEAFY同源基因/启动子/序列分析Key words
Longan/LEAFY gene/Promoter/Sequence analysis分类
农业科技引用本文复制引用
许家辉,朱娜,温超,曾黎辉..龙眼LEAFY同源基因启动子的克隆与序列分析[J].果树学报,2011,28(4):689-693,5.基金项目
国家自然科学基金(30971989) （30971989）
