果树学报2011，Vol.28Issue(4)：610-616,7.
不同用途杏品种群的AFLP分析
Genetic analysis of apricot（Armeniaca） by fluorescent-AFLP markers
摘要
Abstract
In order to determine the genetic structure and genotypic diversity among the different apricot cultivar groups classified according to the use purpose,population genetic structure of 49 apricot accessions was studied using fluorescent-AFLP markers.The results showed that the average number of polymorphic loci was 183.9,the percentage of polymorphic loci was 85.1% by 8 pairs of PstⅠ/ MseⅠprimers in common apricot of five groups.Nei＇s gene diversity（H=0.184）,Shannon information index（I= 0.295）,observed number of alleles（Na=1.851）,and effective number of alleles（Ne=1.294） at species level were higher than ones at group level with significant differences.At group level,the four parameters of the cross-breeding cultivar group were all higher than ones of other four groups.Genetic differentiation coefficient（Gst=0.267 9） for four apricot groups showed that apricot genetic variation was mainly within the groups and accounted for 73.21% of total variations.Nei＇s unbiased measure of genetic identity（IN） and genetic distance（D） were evaluated and the results showed that the genetic similarity between cultivar group for kernel consuming and ornamenting cultivar group was the highest（IN=0.985 0,D=0.015 1）.Compared for other four groups,the cultivar group for flesh and processing was the most close to the cross-breeding cultivar group in genetic distance（IN=0.946 3;D=0.056 5）.AFLP markers were used in cluster analysis with UPGMA and three groups of similar accessions were identified when the coefficient was 0.537：（ⅰ） the group for flesh market and processing,（ⅱ） the proup for kernel consuming and ornamenting cultivar group and（ⅲ） the group including 3 introduced cultivar and 9 cross-breeding accessions.关键词
杏/荧光AFLP标记/遗传多样性/群体遗传结构Key words
Apricot/Fluorescent-AFLP markers/Genotypic diversity/Population genetic structure分类
农业科技引用本文复制引用
张俊环,王玉柱,孙浩元,杨丽..不同用途杏品种群的AFLP分析[J].果树学报,2011,28(4):610-616,7.基金项目
北京市自然基金重点项目(6081001) （6081001）
公益性行业(林业)科研专项(200904032) （林业）
公益性行业(农业)科研专项(201003058) （农业）
