中国生物防治学报2011，Vol.27Issue(3)：349-356,8.
球孢白僵菌对两种植物病原镰孢的拮抗作用
Antagonism of Beauveria bassiana against Two Fusarium spp.Pathogenic to Cotton
摘要
Abstract
In vitro antagonism of Beauveria bassiana,an entomopathogenic fungus,was tested against Fusarium oxysporium f.sp.vasinfecturn,pathogen of cotton wilt,and F.verticillioides,pathogen of cotton red rot.On dual culture,B.bassiana showed obvious inhibition on the growth of the two pathogens.Different doses of conidia had different inhibitory effects,with inhibition rate linearly correlated to inoculum,suggesting that the antagonism was associated with competition for nutrition and space.The inhibition on the two pathogens was both over 90% at inoculum of 6×107 spores·mL-1,slightly higher on F.verticillioide.The metabolite in SDY shake culture of B.bassiana also displayed inhibition,with inhibition rate linearly correlated to metabolite concentration,suggesting that the antagonism was also associated with antibiotic substances.Based on the comparison of inhibition rates,antibiosis was considered to contribute more than competition.In the analysis on the antibiotic metabolite,none of the lyophilized supernatant extracted by 4 different organic extractants showed obvious inhibition on the Fusarium spp.,while addition of crude extract of either polysaccharide or protein significantly inhibited mycelium growth of the two Fusarium spp.,suggesting that the main inhibitory substances are polysaccharide and protein with high molecular weight.关键词
棉花枯萎病/棉花红腐病/球孢白僵菌/拮抗/多糖/蛋白质Key words
cotton wilt disease/cotton red rot disease/Beauveria bassiana/antagonism/polysaccharide/protein分类
农业科技引用本文复制引用
张胜利,李增智..球孢白僵菌对两种植物病原镰孢的拮抗作用[J].中国生物防治学报,2011,27(3):349-356,8.基金项目
国家自然科学基金（30972368) （）
教育部高等学校博士点专项科研基金（200503644001) （）
