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转DREBIA基因多年生黑麦草Tl代萌发期耐盐性研究

包永霞 李雪 满达 韩烈保

草业科学2011,Vol.28Issue(8):1440-1444,5.
草业科学2011,Vol.28Issue(8):1440-1444,5.

转DREBIA基因多年生黑麦草Tl代萌发期耐盐性研究

A study on salt tolerance of T1 generation transgenic perennial ryegrass with DREB1A gene at germination period

包永霞 1李雪 2满达 3韩烈保1

作者信息

  • 1. 北京林业大学草坪研究所,北京100083
  • 2. 北京鼎围昌盛生物技术有限责任公司,北京100083
  • 3. 北京林业大学总务与产业管理处,北京100083
  • 折叠

摘要

Abstract

The seeds of transgenic perennial ryegrass (Loliurn perenne) with DREBIA gene were treated with various concentrations of NaC1 (0, 0.3%, 0.6%, 0.9%, 1.2%and 1.5%) to study physiological re sponses of transgenic plants under salt stresses. The results showed that the seed relative germination potential, relative germination rate, seedling height, leaf chlorophyll content, leaf water content and relative water content (RWC) decreased when NaC1 concentration increased. There were significant differences be tween the transgenic group and control group. Variation of root length was not obvious. The leaf relative conductivity had a positive relationship with the increase of salt concentration and with significant differ ences between transgenic plants and control plants. The salt tolerance of transgenic plants improved com- pared to nontransgenic plants. Transgenic T1 generation seeds of perennial ryegrass with DREB1A gene can be used as seed resources for salt soil improvement.

关键词

多年生黑麦草/DREBlA基因/T1代/萌发期/耐盐性

Key words

Lolium perenne/DREBIA gene/T1 generation/germination stage/salt tolerance

分类

农业科技

引用本文复制引用

包永霞,李雪,满达,韩烈保..转DREBIA基因多年生黑麦草Tl代萌发期耐盐性研究[J].草业科学,2011,28(8):1440-1444,5.

基金项目

北京林业大学研究生科技创新专项计划 ()

北京市重点学科专项 ()

草业科学

OA北大核心CSCDCSTPCD

1001-0629

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