中国动物检疫2011,Vol.28Issue(8):46-50,5.
单增李斯特氏菌溶血素基因的克隆及原核表达
Cloning and Prokaryotic Expression of Hemolysin Gene of Listeria monocytogenes
摘要
Abstract
In this study, the Hly gene ofListeria monocytogenes train C53005 was amplified by PCR using designed primers. Then, the gene was cloned into pMD18-T vector, and indentified by digestion with restriction endonuclease, PCR and sequencing. After the sequenc关键词
单核细胞增生性李斯特氏菌/溶血素基因/克隆/原核表达Key words
Listeria monocytogenes/Hemolysin/Gene cloning/Prokaryotic expression分类
农业科技引用本文复制引用
吴晓薇,徐成刚,马保华,江经纬,叶贺佳,区燕宜,徐小芹,廖明..单增李斯特氏菌溶血素基因的克隆及原核表达[J].中国动物检疫,2011,28(8):46-50,5.基金项目
广东出入境检验检验局科技项目(2011CDK46) ()