陕西师范大学学报：自然科学版2011，Vol.39Issue(4)：39-44,6.
猪肺胞膜窖和窖蛋白分离纯化鉴定及其纳米结构的原子力显微镜研究
Isolation and identification of pig lung Caveolae and research on nanostructure by atomic force microscopy
摘要
Abstract
Caveolae are various invaginations on the surface of cell plasma membrane that related to signal transduction and disease.Caveolae which has unique characteristics of the flotage density ar separated from pig lung by treating with non-ionic detergent TritonX—100 which can not dissolve with caveolae.Gel electrophoresis shows the caveolae relative proteins.Determining the caveolin—1 by dot hybridization shows the caveolae contain caveolin.The protein content is 0.791 13 mg/mL by coomassie blue staining.This method to obtain caveolae and caveolin has some repeatability and stability,which can apply to any biological tissue and cell line.atomic force microscopy（AFM） shows caveolae is 79nm.Then,a new method for isolating caveolae rapidly and completely and identification caveolin by dot hybridization is established.关键词
胞膜窖/SDS聚丙烯酰胺凝胶电泳/点杂交/原子力显微镜Key words
Caveolae/SDS-Polyacrylamide gel electrophoresis/dot hybridization/atomic force microscopy分类
生物科学引用本文复制引用
张勤勤,孙润广,李连启..猪肺胞膜窖和窖蛋白分离纯化鉴定及其纳米结构的原子力显微镜研究[J].陕西师范大学学报：自然科学版,2011,39(4):39-44,6.基金项目
国家自然科学基金资助项目 （）
教育部高校博士点专项科研基金资助项目 （）
