陕西师范大学学报：自然科学版2011，Vol.39Issue(4)：60-64,5.
一氧化氮在萘乙酸诱导不定根形成过程中的作用研究
The role of nitric oxide in 1-naphthylacetic acid-induced adventitious roots formation
摘要
Abstract
The role of nitric oxide（NO） in naphthaleneacetic acid（NAA）-induced adventitious roots formation in mung bean（Phaseolus radiatus L.） hypocotyl cuttings was studied.The results showed that,compared with the control,both NO donor sodium nitroprusside（SNP） and NAA significantly raised number,fresh and dry weight of adventitious roots.The treatment with complex of NAA and SNP resulted in significant increase in adventitious roots number compared with the treatment using SNP or NAA alone.Carboxy-2-phenyl-4,4,5,5-tetramethylimidazoline（c-PTIO）,a NO specific scavenger,effectively inhibited NAA-induced adventitious root formation,and the optimal concentration was 200 μmol/L.Furthermore,inhibitor of nitric oxide synthase（NOS）,N＇-nitro-L-arginine methyl ester（L-NAME） partly inhibited the effect from NAA,the optimal concentration was 30mmol/L.Detection of endogenous NO showed that the level of NO was obviously promoted by NAA,however,innibitd by c-PTIO and L-NAME.All results mentioned above indicated that NO plays a key role in NAA-induced adventitious roots formation,and NOS is involved in the generation of NO in this process.关键词
一氧化氮/一氧化氮合酶/绿豆插条/萘乙酸/不定根形成Key words
nitric oxide/nitric oxide synthase/mung bean cuttings/NAA/adventitious root formation分类
农业科技引用本文复制引用
黄爱霞,佘小平..一氧化氮在萘乙酸诱导不定根形成过程中的作用研究[J].陕西师范大学学报：自然科学版,2011,39(4):60-64,5.基金项目
陕西省自然科学基金青年人才项目 （）
中央高校基本科研业务费专项资金项目 （）
陕西师范大学青年基金资助项目 （）
