陕西师范大学学报：自然科学版2011，Vol.39Issue(4)：69-74,6.
双酚A对中国林蛙精巢芳香化酶和雌激素受体的影响
Effects of bisphenol A on aromatase and estrogen receptor in the testis of frog Rana chensinensis
摘要
Abstract
In order to investigate the effects of bisphenol A（BPA） in water environment on aromatase（P450arom）and estrogen receptor（ER）in the testis of amphibian,adult male frogs Rana chensinensis were exposed to 5×10-7,5×10-8 mol/L BPA for 30 d during spermatogonium proliferation,spermatocyte meiosis and spermatoleosis stages of the testis,respectively.In addition,5×10-9,5×10-10 mol/L 17 β-esradiol（E2） were chosen as positive control.The expressios of P450 arom and ER in testis were detected by immunohistochemistry.The results indicated that P450 arom and ER in Leydig and Sertoli cells of the testis were localized with varying degrees of expression.BPA up-regulates the expression of P450 arom and ER in testis of R.chensinensis during the three stages.There was no significant difference in the inductive effects of bisphenol A on P450 arom,while ER was significant different during the three stages,and the strongest effects were showed at the spermatoleosis stage.These results suggest that the effects of bisphenol A on testicular function were carried out by interfering with P450 arom in sex hormone synthesis pathway,and up-regulating the expression of ER.关键词
双酚A/芳香化酶/雌激素受体/中国林蛙Key words
bisphenol A/P450 arom/estrogen receptor/Rana cahensinensis分类
生物学引用本文复制引用
杨君,张育辉,王宏元,白瑶,洪燕燕..双酚A对中国林蛙精巢芳香化酶和雌激素受体的影响[J].陕西师范大学学报：自然科学版,2011,39(4):69-74,6.基金项目
国家自然科学基金资助项目 （）
