化工进展2011，Vol.30Issue(9)：2049-2054,6.
一种新型壳聚糖分离介质的制备
Study the preparation of a novel crossl-linked chitosan support
摘要
Abstract
Investigation was carried out to elucidate the influence of process parameters on the characteristics of chitosan microspheres prepared by reverse phase suspension cross-linked polymerization.The chitosan droplet solidified and hardened by glutaraldehyde crosslinking agent was dispersed in the oil phase composed of liquid paraffin in the presence of Span 80 surfactant.Iminodiacetic acid（IDA）was attached onto chitosan microspheres activated by epichlorohydrin under basic condition.The results indicated that the optimal epoxy density above 0.15 mmol/g gel was achieved at 10%（Ф） epichlorohydrin as activating accelerator in the solution consisting of 0.6 mol/L NaOH and 40% DMSO.The study on linkaging of IDA demonstrated that the support was synthesized in the solution composed of 0.6 mol/L IDA and 2.0 mol/L NaOH at 60 ℃ for 6 h,whose adsorption of Cu2＋ was up to 172.787 g/g gel.The moisture content of the matrix was up to 45.60% and the porosity was 69.45% under the optimized conditions.The novel matrix could be used in immobilized metal ion affinity chromatography as the solid phase.关键词
壳聚糖/金属螯合层析填料/环氧氯丙烷Key words
chitosan/metal chelate affinity chromatography/epichlorohydrin分类
化学化工引用本文复制引用
易喻,崔国艳,王鸿,李敏,朱克寅,应国清..一种新型壳聚糖分离介质的制备[J].化工进展,2011,30(9):2049-2054,6.基金项目
浙江省科技厅项目 （）
