分析化学2011，Vol.39Issue(7)：998-1002,5.DOI:10.3724/SP.J.1096.2011.00998
基于银纳米粒子构建荧光传感平台用于核酸检测
Silver Nanoparticles as a Fluorescent Sensing Platform for Nucleic Acid Detection
摘要
Abstract
In this study, we reported Ag nanoparticles (AgNPs) as an effective fluorescent sensing platform for nucleic acid detection for the first time. The general concept used in this approach is based on adsorption of the fluorescently labeled single-stranded DNA (ssDNA) probe by AgNP,which is accompanied by substantial fluorescence quenching due to their close proximity, followed by specific hybridization with its target to form a double-stranded DNA (dsDNA). The use of this sensing platform to differentiate complementary and mismatched sequences is also demonstrated.关键词
核酸检测/荧光传感平台/银纳米粒子Key words
Nucleic acid detection/ Fluorescent sensing platform / Silver nanoparticles引用本文复制引用
张瑛洧,李海龙,孙旭平..基于银纳米粒子构建荧光传感平台用于核酸检测[J].分析化学,2011,39(7):998-1002,5.基金项目
本文系中国国家重点基础研究发展计划(No.2011CB935800)资助 （No.2011CB935800）
