广西医学2011，Vol.33Issue(11)：1397-1400,4.
rAd-p53瘤内注射前后鼻咽癌原发灶p53、p21WAF1蛋白的表达及意义
Expression of p53 ,p21WAF1 Protein in Nasopharyngeal Carcinoma Primary Site after rAd-p53 Intratmnoral Injection and its Significance
摘要
Abstract
Objective To investigate the expression of p53 protein and p21WAF1 protein when the recombinant human p53 adenovirus( rAd-p53 )before and after nasopharyngeal primary site intratumoral injection, and to investigate its significance. Methods 51 patients who were diagnosed with advanced nasopharyngeal carcinoma were randomly divided into two groups, treatment group( 27 cases )were treated with tAd-p53 intratumoral injection and concurrent chemo-radiotherapy,and control group( 24 cases )were treated with concurrent chemo-radiotherapy. Clinical data and the nasopharyngeal tumor tissues were collected after intratumoral injection with tAd-p53. And the p53 protein and p21 wan protein expression in the primary tumor tissue of nasopharyngeal carcinoma were detected by immunohistochemistry two step method. Results In treatment group, the p53 protein and p21WAF1 protein expression rates were 33.33％ and 33.3％ before tAd-p53 intratumoral injection and concurrent chemo-radiotherapy,and 62.96％ and 77.77％ after treatment.Both positive expression rate improved obviously after treatment ( P ＜0.05 ). In control group,the p53 protein and p21WAF1 protein expression rates were 40.74％ and 37.5％ respectively before chemo-radiotherapy,and 51.85％ and 50. 0％ after treatment. Both p53 protein and p21wan protein expression heightened after treatment, but there were no statistically significant difference between the two groups( P ＞ 0.05 ). Conclusion The increasing p53 protein expression leads to the increasing expression of p21WAFl protein in nasopharyngeal primary site after tAd-p53 intratumora]injection, which may be the mechanism of nasopharyngeal carcinoma treatment.关键词
鼻咽癌/rAd-p53/p53蛋白/p21WAF1蛋白Key words
Nasopharyngeal carcinoma/ rAd-p53/ p53 protein/ p21WAF1 protein分类
医药卫生引用本文复制引用
黄波,司勇锋,兰桂萍,韦海明,覃扬达,孙金杰,何承诚..rAd-p53瘤内注射前后鼻咽癌原发灶p53、p21WAF1蛋白的表达及意义[J].广西医学,2011,33(11):1397-1400,4.基金项目
广西医药卫生科学研究基金资助项目(重200721 （）
Z2008047) （）
