中华医学杂志（英文版）2011，Vol.124Issue(16)：2540-2547,8.DOI:10.3760/cma.j.issn.0366-6999.2011.16.026
Globular adiponectin protects human umbilical vein endothelial cells against apoptosis through adiponectin receptor 1/adenosine monophosphate-activated protein kinase pathway
Globular adiponectin protects human umbilical vein endothelial cells against apoptosis through adiponectin receptor 1/adenosine monophosphate-activated protein kinase pathway
ZHAO Hong-yu 1ZHAO Min 1YI Tong-ning 2ZHANG Jin3
作者信息
- 1. Department of Emergency, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, China
- 2. Department of Endocrinology, First Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110032, China
- 3. Department of Endocrinology, First Hospital of China Medical University, Shenyang, Liaoning 110001, China
- 折叠
摘要
Abstract
Background Endothelial dysfunction is a key event in the onset and progression of atherosclerosis in diabetic patients.Apoptosis may lead to endothelial dysfunction and contribute to vascular complications. However, no study has addressed apoptosis in human umbilical vein endothelial cells (HUVECs) induced by an intermittent high-glucose media and its association with adiponectin receptor 1 (adipoR1), adipoR2, or adenosine monophosphate (AMP)-activated protein kinase (AMPK).Methods HUVECs were cultured in continuous normal glucose (5.5 mmol/L), continuous high glucose (25 mmol/L),alternating normal and high glucose and mannitol. In the alternating normal and high-glucose media, HUVECs were treated under different conditions. First, cells were transfected with the adipoR1-specific small-interfering RNA (siRNA)and then stimulated with globular adiponectin (gAD). Second, cells were cultured in both gAD and the AMPK activator 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR). Third, cells were cultured in the AMPK inhibitor adenine-9-β-D-arabino-furanoside (araA), gAD, and in AICAR.Results HUVEC apoptosis increased more significantly in an intermittent high-glucose medium than in a constant high-glucose medium. HUVEC apoptosis induced by an intermittent high-glucose medium was inhibited when the cells were pretreated with 3 μg/ml gAD, which rapidly activated AMPK and adipoR1 in HUVECs. However, adipoR2 was not activated.Conclusions We found that adipoR1, not adipoR2, is involved in mediating intermittent high-concentration glucoseevoked apoptosis in endothelial cells. gAD activated AMPK through adipoR1, leads to the partial inhibition of HUVEC apoptosis. A fluctuating glucose medium is more harmful than a constant high-glucose medium to endothelial cells.关键词
globular adiponectin/adiponectin receptor 1/AMP-activated protein kinase/apoptosis/intermittent glucoseKey words
globular adiponectin/adiponectin receptor 1/AMP-activated protein kinase/apoptosis/intermittent glucose引用本文复制引用
ZHAO Hong-yu,ZHAO Min,YI Tong-ning,ZHANG Jin..Globular adiponectin protects human umbilical vein endothelial cells against apoptosis through adiponectin receptor 1/adenosine monophosphate-activated protein kinase pathway[J].中华医学杂志（英文版）,2011,124(16):2540-2547,8.
