首页|期刊导航|中华医学杂志（英文版）|Amyloid β3-10 DNA vaccination suggests a potential new treatment for Alzheimer's disease in BALB/c mice
中华医学杂志（英文版）2011，Vol.124Issue(17)：2636-2641,6.DOI:10.3760/cma.j.issn.0366-6999.2011.17.013
Amyloid β3-10 DNA vaccination suggests a potential new treatment for Alzheimer's disease in BALB/c mice
Amyloid β3-10 DNA vaccination suggests a potential new treatment for Alzheimer's disease in BALB/c mice
摘要
Abstract
Background Amyloid β1-42 (Aβ42) peptide vaccination has been proved to be effective in reducing amyloid burden in brain and improving cognitive function in Alzheimer's disease (AD) mouse models.But the phase Ⅱ trial of Aβ42 peptide vaccine was halted because of T cell-mediated meningoencephalitis.In this study,a DNA vaccine,p(Aβ3-10)10-CpG,was constructed to test whether it would induce predominant TH2 immune response upon immunization of BALB/c mice.Methods BALB/c mice were vaccinated intramuscularly with p(Aβ3-10)10-CpG plasmids.Aβ42 peptide,pcDNA3.1(+)empty vector and PBS were injected to the control groups.Expression of interesting gene in injected muscle was identified by immunohistochemistry.Anti-Al3 antibody titers,isotype profiles as well as cytokines in ex vivo splenocytes culture supernatants were analyzed by enzyme-linked immunosorbent assay (ELISA).Results P(Aβ3-10)10-CpG plasmid was expressed in muscle after injection detected by immunohistochemistry.The p(Aβ3-10)10-CpG vaccine induced high titers of anti-Aβ antibodies in BALB/c mice.And isotype of the antibodies was mainly IgG1,the IgG1/IgG2a ratio for the p(Aβ3-10)10-CpG group was approximately 5 times greater than that for the Aβ42 peptide group.Ex vivo cultured splenocytes isolated from mice immunized with p(Aβ3-10)10-CpG exhibited high interleukin-4 response and low interleukin-Y (IFN-Y) response.Conclusions Immunization with p(Aβ3-10)10-CpG vaccine primarily induces a TH2 type of response,thus reduces the probability of inflammation.This p(Aβ3-10)10-CpG vaccine possesses the basic factors required for a safe and effective AD vaccine.关键词
Alzheimer's disease/vaccine/DNAKey words
Alzheimer's disease/vaccine/DNA引用本文复制引用
XING Xiao-na,ZHANG Wei-ge,SHA Sha,LI Yu,GUO Rong,WANG Cai,CAO Yun-peng..Amyloid β3-10 DNA vaccination suggests a potential new treatment for Alzheimer's disease in BALB/c mice[J].中华医学杂志（英文版）,2011,124(17):2636-2641,6.基金项目
This study was supported by a grant of the National Natural Science Foundation of China (No.30471927). （No.30471927）
