中华医学杂志（英文版）2011，Vol.124Issue(16)：2534-2539,6.DOI:10.3760/cma.j.issn.0366-6999.2011.16.025
Perfluorocarbon attenuates lipopolysaccharide-mediated inflammatory responses of alveolar epithelial cells in vitro
Perfluorocarbon attenuates lipopolysaccharide-mediated inflammatory responses of alveolar epithelial cells in vitro
XU Shu-feng 1CHEN Liang-an 2WANG Ping 1LIU Rui-ji 1ZHAO Jing 2ZHANG Xiang-ning 2FU Zhan-zhao 2GAO Li-ming 2LIANG Zhi-xin 2SUN Ji-ping1
作者信息
- 1. Department of Respiratory Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, China
- 2. Department of Respiratory Medicine, the First Hospital of Qinhuangdao, Qinhuangdao, Hebei 066000, China
- 折叠
摘要
Abstract
Background Toll-like receptor-4 (TLR-4) is integrally involved in lipopolysaccharide (LPS) signaling and has a requisite cytoprotective effect have yet to be elucidated. Therefore we examined in an in vitro model the cytoprotective effect of PFC on LPS-stimulated alveolar epithelial cellls (AECs).Methods AECs (A549 cells, human lung adenocarcinoma cell line) were divided into four groups: control, PFC, LPS and LPS + PFC (coculture group) groups. Intercellular adhesion molecule-1 (ICAM-1) was detected by ELISA, tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) were detected by radioimmunological methods. The expression of Results ICAM-1, TNF-a and IL-8 were significantly increased in LPS-stimulated AECs groups. The expression of translocation into the nucleus. There were no significant effects of PFC alone on any of the factors studied while the coculture group showed significant downregulation of the secretion of ICAM-1, TNF-α and IL-8, the expression of TLR-4 Conclusions Taken together, our results demonstrate that LPS can induce AEC-related inflammatory injury via the injury by blocking the initiation of the LPS signaling pathway, which is indicated by the significant decrease of TLR-4关键词
perfluorocarbon/lipopolysaccharide/Toff-like receptor-4/nuclear factor-кB/alveolar epithelial cellsKey words
perfluorocarbon/lipopolysaccharide/Toff-like receptor-4/nuclear factor-кB/alveolar epithelial cells引用本文复制引用
XU Shu-feng,CHEN Liang-an,WANG Ping,LIU Rui-ji,ZHAO Jing,ZHANG Xiang-ning,FU Zhan-zhao,GAO Li-ming,LIANG Zhi-xin,SUN Ji-ping..Perfluorocarbon attenuates lipopolysaccharide-mediated inflammatory responses of alveolar epithelial cells in vitro[J].中华医学杂志（英文版）,2011,124(16):2534-2539,6.
