口腔颌面外科杂志2011，Vol.21Issue(4)：256-260,5.DOI:10.3969/j.issn.1005-4979.2011.04.006
细胞-膜片复合珊瑚支架修复兔颅骨极限缺损的实验研究
Evaluation of a Hybrid Scaffold/Cell Construction Repairing Critical Size Defect in Rabbits
黄辉 1张森林 2刘向辉 1张林 1张纯1
作者信息
- 1. 解放军第81医院口腔科,江苏,南京,210002
- 2. 南京军区南京总医院口腔科,江苏,南京,210002
- 折叠
摘要
Abstract
Objective: The purpose of this study was to evaluate the efficacy of a hybrid scaffold/cell construction repairing bone defects in rabbits.Methods: Mesenchymal stromal cells (MSCs) suspended in 500μl medium was dissolved in platelet rich plasma (PRP).The mixture of MSCs and PRP was dripped into one 6-well plate and further mixed with prothrombin solution and cultured in a humidified incubator for 3 weeks.The culture medium was standard DMEM for the first week.Osteogenic DMEM was used as the culture medium for next two weeks.The medium was changed every other day.MSCs sheet formed and were detached intactly from the substratum using a cell scraper.The cell-sheet was then wrapped around the coral scaffold and the hybrid sheet-scaffold construct was implanted into a 15mm-diameter rabbit calvarial defect.Autograft and coral implants acted as controls.The new bone formation was investigated by gross examination, histological observation and histomorphometrie analyses in 8- and 16-week after operation.Results: Eight weeks after grafting, new bone in the test group was distributed throughout the coral scaffolds and its surface.New bone in the coral implant group was appeared only in the periphery region.Histomorphometrie data was revealed a significantly higher bone area in test group than in the coral group (P＜0.01).The bone defects of the test group were repaired completely with new bone at the end of sixteen weeks after grafting, while those of the coral group were repaired partly with bone, and fibrous tissue was evident in the central region of defects.The test group had obvious advantage over the coral group in term of bone regeneration (P＜0.01).There was no significant difference in bone formation between the test group and autograft group at 16 weeks after grafting (P＞0.05).Conclusion: The method of growing cell-sheet by combined MSCs and PRP is convenient and simple.The bone graft could bo engineered through combination of the sheet and coral scaffold.关键词
骨组织工程/富血小板血浆/骨髓基质细胞/珊瑚/细胞膜片技术/颅骨极限缺损Key words
bone tissue engineering/ platelet-rich plasma/ marrow stromal cells/ coral/ cell-sheet technology/ critical calvarial defect分类
医药卫生引用本文复制引用
黄辉,张森林,刘向辉,张林,张纯..细胞-膜片复合珊瑚支架修复兔颅骨极限缺损的实验研究[J].口腔颌面外科杂志,2011,21(4):256-260,5.
