极地研究2011，Vol.23Issue(2)：108-114,7.DOI:10.3724/SP.J.1084.2011.00108
一株分离自北极海冰的产β-半乳糖苷酶的海单胞菌(Marinomonas sp. BSi20414)的筛选、鉴定和产酶条件研究
PRELIMINARY STUDIES ON THE SCREENING, IDENTIFICATION AND OPTIMUM FERMENTATIVE CONDITIONS OF A STRAIN MARINOMONAS SP. BSI20414 ISOLATED FROM ARCTIC SEA ICE PRODUCING β-GALACTOSIDASE
摘要
Abstract
A strain BSi20414 with higher β-galactosidase activity was isolated from Arctic sea ice. On the basis of its Physiological and biochemical characteristics and 16S rDNA sequence, it was identified as Marinomonas sp. BSi20414. A series of detailed experiments on the conditions ofβ-galactosidase production were conducted and the results showed that the optimal fermentation conditions for enzyme production were 30℃, 1.5％ (w/v) lactose, initial pH 7.0, 3％ inoculum,80 mL fermentative medium in a 500 mL flask, and 180 rpm for 96 hrs. Under these conditions, the enzyme activity of 1.01 U/mL in the second screening stage increased to 2.92 U/mL. Preliminary experiments showed that the optimal reaction temperature of the crude enzyme on ONPG substrate was 60℃. This result suggests that this cold-adapted strain Marinomonas sp. BSi20414 produces a high-temperature enzyme.关键词
海单胞菌/β-半乳糖苷酶/产酶条件Key words
Marinomonas/ β-galactosidase/ fermentation conditions引用本文复制引用
曾倩,王毓舒,孙鲲,俞勇,陈波..一株分离自北极海冰的产β-半乳糖苷酶的海单胞菌(Marinomonas sp. BSi20414)的筛选、鉴定和产酶条件研究[J].极地研究,2011,23(2):108-114,7.基金项目
海洋公益性行业科研专项经费项目(201005032-4)、中国极地科学战略研究基金(200601)和南北极环境综合考察专项研究项目资助 （201005032-4）
