内科理论与实践2011，Vol.6Issue(1)：52-55,4.
黄芩苷诱导Burkitt淋巴瘤细胞凋亡的初步研究
A preliminary study on baicalin induced apoptosis in human B-lymphoma cell line
摘要
Abstract
Objective To investigate the effect of baicalin on inducing apoptosis in Burkitt's lymphoma cell line Daudi cells. Methods Human Burkitt's lymphoma cell line Daudi cell was incubated with baicalin. Cell growth inhibition was estimated by 3-(4,5)-dimethylthiahiazo(-z-yl)-di-phenytetrazoliumromide(MTT) test Cell morphology was evaluated by light microscopy with Wright's staining. Cell apoptosis was analyzed by flow cytometry. The expressions of caspase-3, caspase-9 were determined by Western blot Results The half maximal inhibitory concentration (IC50) of baicalin on Daudi cells at 72 h was (10.1 ±0.5) μg/mL. The inhibitory effect on cell growth was dose-dependent Morphological study revealed obvious apoptotic cells. Flow cytometry at 48 hours showed that the percentages of apoptosis in baicalin 40 μg/mL and 20 μg/mL groups were 61.5％±6.3％ and 44.2％±4.7％, respectively, remarkably higher than that in control group (8.2％:±0.9％, P＜0.01).The expressions of procaspase-3 and -9 decreased while the expression of activatied caspase-3 was up-regulated in a timedependent manner (24, 36 h). Conclusions Baicalin could efficiently inhibit Burkitt's lymphoma cell proliferation and induced cell apeptosis. The abevementioned caspases might be involved in the baicalin induced Daudi cell apoptosis process.关键词
黄芩苷/Burkitt淋巴瘤/细胞凋亡Key words
Baicalin/ Burkitt lymphoma/ Apoptosis分类
医药卫生引用本文复制引用
肖丹,朱伟嵘,赵维莅..黄芩苷诱导Burkitt淋巴瘤细胞凋亡的初步研究[J].内科理论与实践,2011,6(1):52-55,4.基金项目
国家高技术研究发展计划(项目编号:2006AA02A301):霍英东青年教师基金(项目编号:111035):上海市基础研究重点项目(项目编号:08JC141500):上海自然科学基金(项目编号:08411953900):上海市科委启明星跟踪计划(项目编号:09QH1401700) （项目编号:2006AA02A301）
上海市教委曙光计划(项目编号:09SG21) （项目编号:09SG21）
