现代检验医学杂志2011，Vol.26Issue(4)：103-104,107,3.DOI:10.3969/j.issn.1671-7414.2011.04.035
荧光法测定同型半胱氨酸的方法学评价
Analytical Evaluation of the Novel Biochemical Assay for Homocysteine
邵坤宁 1杨丽娟 1张海燕1
作者信息
- 1. 清华大学第一附属医院糖尿病研究所,北京,100016
- 折叠
摘要
Abstract
Objective To evaluate the features of total Homocysteine (Hcy) assay using novel biochemical assay. Methods Performance characteristics such as precision,linearity,interference testing were evaluated respectively according to EP5-A,EP6-A and EP7-A issued by the National Committee on Clinical Laboratory Standards(NCCLS). Results The CVs of within-run and total precisions at high concentration leveI(Hcy 20.0 μmol/L) were 1. 88％ and 3. 71％ respectively,CVs of within-run and total precisions at low concentration level(Hcy 10. 0 μmol/L) were 2. 76％ and 3. 98％ respecrively. The linear range was 0～60. 0 μmol/L. When the concentration of bilirubin was at 500 μmol/L or triacylglyceride at 15. 0 mmol/L or vitamin C at 10. 0 mmol/L or hemoglobin at 16. 0 g/L produced interference deviation was less than 5 ％. Correlation bewteen results obtainedby this method and hplc method was well,decisive coefficient (r2) =0. 976 1. Conclusion Performance of precision,linearity and interference testing of the homocysteine assay kit produced by XingNen Company could fulfill the requirements of clinical laboratory.关键词
同型半胱氨酸/荧光法Key words
homocysteine/novel assay分类
医药卫生引用本文复制引用
邵坤宁,杨丽娟,张海燕..荧光法测定同型半胱氨酸的方法学评价[J].现代检验医学杂志,2011,26(4):103-104,107,3.
