色谱2011，Vol.29Issue(7)：631-636,6.DOI:10.3724/SP.J.1123.2011.00631
液相色谱/四极杆-飞行时间质谱法筛查奶酪中29种禁用和限用合成色素
Screening method for 29 forbidden or limited synthetic pigments in cheese by liquid chromatography/quadrupole time-of-flight mass spectrometry
摘要
Abstract
A screening method for 29 forbidden or limited synthetic pigments in cheese samples was established by liquid chromatography/quadrupole time-of-flight mass spectrometry ( LC/Q-TOF MS ). The pigments were extracted by n-hexane/water ( 3∶1 , V/V ). After extraction, the n-hexane extract, water extract and residue, were obtained. The n-hexane extract was then cleaned-up by gel permeation chromatography ( GPC ). The water extract was extracted by acetonitrile. and the residue by ammonia water/methanol ( 1 ∶99. V/V ). The results showed that the 29 synthetic pigments with a wide range of polarities were extracted effectively with the recoveries between 70％ and 95％ . and matched well by Q-TOF MS precision mass searching to the mass spectral library with matching scores between 59.66 and 99.47. The quantitative analysis of the 29 pigments was carried out by Target MS/MS. The limits of detection ( LODs ) for 8 Sudan dyes were 0.4 - 2.5 μg/kg while for 21 water-soluble synthetic pigments were 20 - 80 μg/kg. The screening method is suitable for a wide range of synthetic pigments, and can be applied to food samples with proteins and fat in matrix.关键词
液相色谱/四极杆-飞行时间质谱/合成色素/奶酪/筛查Key words
liquid chromatography/quadrupole time-of-flight mass spectrometry ( LC/Q-TOF MS )/ synthetic pigment/ cheese/ screening分类
化学化工引用本文复制引用
赵延胜,杨敏莉,张峰,冯峰,储晓刚,董英..液相色谱/四极杆-飞行时间质谱法筛查奶酪中29种禁用和限用合成色素[J].色谱,2011,29(7):631-636,6.基金项目
北京市科技计划项目(D08050200310803)和国家质量监督检验检疫总局科技计划项目(2010IK138). （D08050200310803）
