重庆医学2011，Vol.40Issue(26)：2647-2648,2651,后插2,4.DOI:10.3969/j.issn.1671-8348.2011.26.021
非甾体类抗炎药NAG-1在胃癌中表达及意义
Expression of nonsteroidal anti-inflammatory drug-activated gene-1 in human gastric cancer tissue and its significance
聂胜峰 1兰斌 2张军 2曾志峰 1丁洁3
作者信息
- 1. 江西省新余市人民医院普外科,338000
- 2. 福建医科大学附属第一医院胃肠外科二区,350005
- 3. 江西省新余市人民医院检验科,338000
- 折叠
摘要
Abstract
Objective To study the expression of nonsteroidal anti inflammatory drug - activated gene-1 in human gastric cancer and its relationship with the clinical staging,differentiation degree,and lymphatic metastasis of gastric cancer.Methods The expression of NAG-1 was detected by semiquantitative reverse transcriptase polymerase chain Reaction ( RT-PCR) and immunohistochemistry(IHC) in the cancer tissues and corresponding adjacent normal tissues in 76 gastric cancer patients.Results Immuno-histochernistry showed the expression of NAG-1 protein in the cancer tissues was lower than that in the normal tissues (x2 = 47.186 ,P＜0.05) ; and the expression was relationship with differentiation degree( x2 = 7.506 ,P＜0.05) , but the expression was not associated with the depth of tumor invasion,lymph node metastasis, and clinical stage(all P＞0.05).The result of RT-PCR showed that NAG-1 mRNA expression in gastric cancer tissue significantly was lower than that in normal tissues(P＜0.05).NAG-1mRNA expression in well and moderately differentiated cancer cells was significantly higher than those of poorly and non-differerntiated cancer cells(P＜0.05) , the expression was not associated with the depth of tumor invasion, lymph node metastasis, and clinical stage(P＞0.05).Conclusion Decreased expression of NAG-1 in human gastric cancer,and it may play an important role in the tumorigenesis of gastric cancer.关键词
胃癌/逆转录聚合酶链反应/免疫组织化学/非甾体抗炎药活化基因-1Key words
gastric neoplasms / reverse transcriptase polymerase chain reaction / immunohistochemistrv/ NAG-1引用本文复制引用
聂胜峰,兰斌,张军,曾志峰,丁洁..非甾体类抗炎药NAG-1在胃癌中表达及意义[J].重庆医学,2011,40(26):2647-2648,2651,后插2,4.
