山东医药2011，Vol.51Issue(32)：26-28,后插3,4.
黄芪多糖对2型糖尿病患者外周血内皮祖细胞增殖及NO生成的影响
Effect of astragalus polysaccharides on proliferation and NO production of endothelial progenitor cells from peripheral blood in patients with type 2 diabetes
摘要
Abstract
Objective To observe the effect of astragalus polysaccharides ( APS) on proliferation and nitric oxide (NO) production of endothelial progenitor cells ( EPCs) from peripheral hlood in patients with type 2 diabetes mellitus (T2DM).Methods Total mononuclear cells were isolated by Ficoll density gradient centrifugation.After 7 days of culture, EPCs were characterized , the effect of APS on EPCs proliferation was ohserved.EPCs were treated with various concentration and different time culture of APS, then treated with LY294002 ( PI3K inhibitor) , then the NO level was measured.Results APS could promote the proliferation of EPCs in T2DM, and increase the endothelial NO production in a dose and a time-dependent manner.LY294002 prevented the NO production induced by APS.Conclusions The rat tail collagen can be a more cheaper replacement of the fibronectin to culture the EPCs from peripheral blood in patients with T2DM.Treatment with APS can increase the NO production and markedly promote the proliferation of EPCs in T2DM,LY294002 prevents the NO production induced by APS.关键词
黄芪多糖/糖尿病,2型/内皮祖细胞/增殖/一氧化氮Key words
astragalus polysaccharides/ diabetes mellitus, type 2/ endothelial progenitor cells/ proliferation/ nitric oxide分类
医药卫生引用本文复制引用
徐寒松,吴青,谢晓云,孔德明..黄芪多糖对2型糖尿病患者外周血内皮祖细胞增殖及NO生成的影响[J].山东医药,2011,51(32):26-28,后插3,4.基金项目
国家自然科学基金资助项目(30960491) （30960491）
贵州省优秀科技教育人才省长基金资助项目[黔省专合字(2009)84号]. （2009）
