山东医药2011，Vol.51Issue(32)：29-32,4.
运用消减杂交技术构建IgA肾病肾阳虚证的相关基因文库
Suppression subtractive hybridization to obtain differential genes associated kidney yang defficiency of IgA nephropathy
摘要
Abstract
Objective To construct cDNA substractive library of kidney yang defficiency of IgA nephropathy ( IgAN)by suppression subtractive hybridization ( SSH).Methods The research related genes of kidney yang defficiency syndrome of IgAN.Microamount RNA was amplified.SSH and gene cloning were used to obtain differential genes associated kidney yang defficiency of IgAN.Result A Total of 537 clones were obtained.276 from the forward subtractive library and 261 from the reverse subtractive library.Conclusion The cDNA subtractive library is successfully constructed and it may provide a solid foundation for screening and cloning the genes of kidney yang defficiency.关键词
肾小球肾炎,IgA/肾阳虚/抑制性消减杂交/cDNA消减文库Key words
glomerulonephritis, IgA/ kidney yang defficiency/ suppression suhtractive hybridization/ cDNA subtractive lihrary分类
医药卫生引用本文复制引用
李玉萍,罗仁,孙晓敏,聂晓莉,赵晓山,杨海文..运用消减杂交技术构建IgA肾病肾阳虚证的相关基因文库[J].山东医药,2011,51(32):29-32,4.基金项目
广东省自然基金博士启动项目(9451051501002788). （9451051501002788）
