山东医药2011，Vol.51Issue(32)：36-38,3.
HCV NS5A结合蛋白顺乌头酸酶1的验证研究
Confirm the HCV NS5A protein interaction with aconitase 1
王晓杰 1相久全 2韩乐强 3张锦前 1成军1
作者信息
- 1. 首都医科大学附属北京地坛医院,北京,100015
- 2. 密云县第二医院
- 3. 大连市第六人民医院
- 折叠
摘要
Abstract
Objective To screen proteins of human liver cDNA library interacting with HCV NS5A and confirm the interaction between HCV N55A protein and aconitate 1 protein.Methods Yeast two hybrid was performed by mating AH109 ( HCV NS5A) with Y187 ( liver cDNA lihrary of human) .The interacted proteins with HCV NS5A were screened from the library.The aconitate 1 gene was amplified at first.The expression vectors of aconitate 1 were constructed.Then the interaction between HCV N55A protein and Homo sapiens aconitate 1 protein was confirmed using co-imruunoprecipitation and mammalian two hybrid experiment.Results The eukaryotic expression vectors of aconitate 1 were constructed.These results showed that Homo sapiens aconitate 1 protein interacted with HCV NS5A protein by co-immunoprecipitation and mammalian two hybrids experiment Conclusion HCV NS5A and Homo sapiens aconitate 1 protein can interact in HepCJ2 cells.关键词
HCV NS5A/顺乌头酸酶1/酵母双杂交/哺乳动物双杂交/免疫共沉淀Key words
hepatitis C virus NS5A/ aconitate 1 / yeast two hybrid/ mammalian two bybrid/ co-immunoprecipitation分类
医药卫生引用本文复制引用
王晓杰,相久全,韩乐强,张锦前,成军..HCV NS5A结合蛋白顺乌头酸酶1的验证研究[J].山东医药,2011,51(32):36-38,3.
