山东医药2011，Vol.51Issue(30)：15-17,3.
NF-κB信号传导通路相关基因不同表型肝癌患者尿8-OHdG水平观察
Difference of urinary 8-OHdG levels among varied genotypes of NF-KB signaling pathway genetic polymorphismin in hepatocellular carcinoma patients
摘要
Abstract
Objective To investigate the difference of urinary 8-OHdG levels among varied genotypes of NF-kB signaling pathway genetic polymorphisms in hepatocellular carcinoma patients. Methods TNF-α, NF-kB, iNOS, COX-2 phenotype of NF-kB signaling pathway in 155 cases of hepatocellular carcinoma ( HCC group) were detected by PCR-RFLP method. Urinary 8-OHdG levels in HCC group and control groups were detected by ELISA. Results Urinary 8-OHdG levels in HCC group was (9.29 ± 8.04) ng/mg Ct, and control group was ( 7.27 ± 5.06 ) ng/mg Ct, respectively ( P ＜0. 05 ). And no significant difference of urinary 8-OHdG levels had been detected among different polymorphism genotypes in TNF-α (G-308A), NF-kB (-94ins/delATTG), iNOS (C608T) and COX-2 (T8473C). Conclusions Urinary 8-OHdG levels in hepatocellular carcinoma tissue are higher than normal. Genetic polymorphisms of NF-kB signaling pathway are not associated with urinary 8-OHdG levels in hepatocellular carcinoma patients.关键词
肝癌/核因子κB信号传导通路/基因多态性/DNA氧化损伤Key words
hepatocellular carcinoma/ NF-kB signaling pathway/ genetic polymorphism/ oxidative DNA damage分类
医药卫生引用本文复制引用
唐耘天,刘天奇,杨建荣,罗建强,梁中骁..NF-κB信号传导通路相关基因不同表型肝癌患者尿8-OHdG水平观察[J].山东医药,2011,51(30):15-17,3.基金项目
广西壮族自治区自然科学基金项目(0897011). （0897011）
