重庆医学2011，Vol.40Issue(25)：2546-2548,3.DOI:10.3969/j.issn.1671-8348.2011.25.022
次乌头碱对心肌细胞Ca2+调控蛋白mRNA表达的影响
Effects of hypaconitine on expression of mRNA of calcium regulated proteins in myocardial cells
摘要
Abstract
Objective To observe the effects of Hypaconitine( HA)on mRNA expression of calcium regulated proteins such as L-type calcium channel α1C, calmodulin(CaM) and RyR2 in myocardial cells.Methods Primary neonatal rat myocardial cells were generated and cultured.L-type calcium channel α1C,CaM and RyR2 mRNA were measured by transcription-polymerase chain reaction(RT-PCR) respectively.Results After coincubating with HA for 15 min,60,120 μM/L HA can improve the expression of Ltype calcium channel α1C mRNA in myocardial cells (P＜0.05) , 30 , 60 , 120 μM/L HA also can increase CaM mRNA in myocardial cells(P＜0.01) ,60, 120 μM/L HA increased the expression of RyR2 mRNA in myocardial cells(P ＜0.05).After coincubation with HA for 5,15,60 min, 60 μM/L HA increased the expression of L-type calcium channel α1C mRNA of myocardial cells in 5min(P＜0.05).The expression of CaM mRNA of myocardial cells were increased after coincubating with 30 , 60 μM/L HA for 15 ,60 min(P＜0.01).Conclusion HA(＞60 μM/L)can induce the calcium overload in myocardial cells, which will result in arrhythmia.This action maybe relates to the increasing expression of L-type calcium channel α1C,CaM and RyR2 mRNA of myocardial cells caused by HA.The clinical toxic arrhythmias can be relieved through tnterfering these target genes when toxic reaction happens.关键词
次乌头碱/心肌细胞/钙调控蛋白Key words
hypaconitine/ mvocardial cells / calcium regulated proteins引用本文复制引用
李志勇,谭鹏,赵晖,李飞,孙建宁..次乌头碱对心肌细胞Ca2+调控蛋白mRNA表达的影响[J].重庆医学,2011,40(25):2546-2548,3.基金项目
国家科技部十五科技攻关计划重大项目(2004BA721A11) （2004BA721A11）
国家973计划资助项目(2009CB522800). （2009CB522800）
